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This technique is similar to the previous one up to the laboratory phase, where fertilization is carried out by means of “Intracytoplasmic Sperm Injection” (ICSI).

Inyección intracitoplasmática de espermatozoides, fecundación in vitro

IVF technique using ICSI

Microinjection will be carried out on oocytes that have a correct maturation (approximately 80%), that is, those that are in the Metaphase II stage. In order to do this, we have to free them from the cells that surround them. This process is known as “denudation”.

By means of a micromanipulator we will carry out the sperm microinjection. The microinjector consists of an inverted microscope (with which we can observe oocytes and sperm with up to 400 magnifications) and microinjector arms. With one of them, the oocyte is attached to the microinjector by suction, and with the other hydraulic arm, the sperm is captured and introduced into the oocyte, thus facilitating fertilization.

The method

Subsequently the microinjected oocytes are kept in conditions as similar as possible to physiological ones. This is achieved by keeping the oocytes in an incubator, with a stable temperature of 37º C and 6% CO2, until their fertilization is checked 16-18 hours after ICSI. After two or three days (day +2 or +3) after fertilization (ICSI), the pre-embryos are transferred to the interior of the uterus, and if there are supernumerary pre-embryos, they can be cryopreserved for another transfer, thus avoiding having to undergo stimulation again. In both techniques, if we obtain supernumerary pre-embryos, these can be kept in “prolonged culture” up to the stage of expanded blastocyst, performing the transfer in day +5 or +6, allowing us a better embryo selection, and therefore a greater number of pregnancies.

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Written by: Sabrina Camara